Plasmid

Part:BBa_K105000:Design

Designed by: Manuel Gersbacher, Robin Sorg, Marius Müller, Paul Bourgine, Sandra Dittmann, Michi Wild und Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-10-28)


pSB1A2_fusion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2057
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2057
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2057
    Illegal XbaI site found at 2072
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1096


Design Notes

To enable the fusion with other domains in frame this vector has no base pair in between the restriction sites and the BioBrick. <br\n>

For more information about this issus, see:<br\n>

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).



Source

pSB1A2 + inserts without bases between the BioBrick and the restriction sites

References